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2BI261 Biochemistry and Molecular Biology lab

Learning outcomes

The candidate having completed the course have the following learning outcomes:

Skills

The Candidate can

  • master laboratory methods central to the fields of biochemistry and molecular biology, and use of the principles that underlie each method

Competence

The Candidate

  • can work independently with the techniques and instrumentation employed in the course

Course content

 

  • Biochemistry: Quantitative measurement, spectrophotometry, isolation and characterisation of DNA and proteins, ion exchange chromatography, SDS-PAGE, enzyme kinetics, carbohydrate chemistry.
  • Molecular Biology: Polymerase chain reaction (PCR), gel electrophoresis, cloning into plasmid vectors, preparation & transformation of competent E. coli, plasmid DNA isolation, restriction analysis, DNA cycle sequencing & capillary electrophoresis, bioinformatics. 

 

Teaching and working methods

 

  • group work in the laboratory
  • submission of laboratory reports of completed exercises
  • student presentations
  • independent study groups.

Coursework requirements

 

Approved laboratory reports from all the exercises for which a report is required, 7 in Biochemistry and one in Molecular Biology.

Examination

 

  • individual portfolio (includes two reports from the Biochemistry section, selected by the instructor and one comprehensive report from Molecular Biology section), counts 60 %
  • individual written examination, 4-hour, counts 40 %

Both parts must be passed to receive a final grade.

The grading system is based on the letters from A-F, where E is the minimum passing grade.